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Sanger Sequencing

As Letgen Biotechnology, we are also offering Sanger Sequencing service along with NGS and primer/probe design services. This method, which was developed by Frederick Sanger and his colleagues in 1977, allows us to identify nucleotide sequences in DNA with an approach based on a “chain termination” philosophy.

Although Next Generation Sequencing methods are used for large-scale sequences today, Sanger Sequencing still maintains its place as a frequently used method.

It has mainly 3 steps which can be listed as;

  • PCR
  • Size Separation by Gel Electrophoresis
  • Gel Analysis & Determination of DNA Sequence