As Letgen Biotechnology, we are also offering Sanger Sequencing service along with NGS and primer/probe design services. This method, which was developed by Frederick Sanger and his colleagues in 1977, allows us to identify nucleotide sequences in DNA with an approach based on a “chain termination” philosophy.
Although Next Generation Sequencing methods are used for large-scale sequences today, Sanger Sequencing still maintains its place as a frequently used method.
It has mainly 3 steps which can be listed as;
- Size Separation by Gel Electrophoresis
- Gel Analysis & Determination of DNA Sequence