Liquid biopsy is a non-invasive diagnostic method that has revolutionized many fields from cancer to neurological diseases. Liquid biopsy materials obtained from samples such as plasma, serum, urine and cell culture media have great potential for early diagnosis and monitoring of diseases. However, RNA from these samples, especially small extracellular RNA (cfRNA), is present in extremely low concentrations. This can lead to target RNA being missed during RNA isolation. Furthermore, background noise, which inevitably occurs in liquid biopsy samples, complicates the RNA sequencing process and poses serious challenges for analysis and interpretation.
Small RNA Sequencing(Small-seq) Problems Encountered
Small RNA is critical for the study of small RNA molecules, especially microRNAs. However, a number of problems are encountered in this process:
1. Background Noise: Background noise from unwanted sources can mask the true biological signals. This is particularly pronounced in liquid biopsy samples due to the low concentration of RNA.
2. High Proportion of Unmapped Sequences: A significant proportion of the reads obtained during sequencing cannot be accurately mapped on the reference genome. That is, these reads are sequences that cannot be identified where they belong in the genome. This is particularly common in liquid biopsy samples due to low RNA concentration and background noise. Unmapped sequences degrade data quality and make it difficult to detect true biological signals. This prevents researchers from obtaining meaningful and reliable results.
3. Wasted Sequencing Capacity: Some of the reads obtained during sequencing do not carry biologically meaningful information. These “meaningless reads” result in wasted sequencing capacity. For example, if a limited number of reads can be taken in a sequencing run and some of these reads are due to background noise or contamination, this is an inefficient use of sequencing capacity. As a result, additional sequencing runs may be necessary to obtain a sufficient amount of meaningful data. This requires more reagents, time and labor, increasing costs and slowing down the research process.
EXTRAClean Series: A Revolutionary Solution
Norgen Biotek’s EXTRAClean series is specifically designed to address these challenges in RNA isolation from liquid biopsy samples and small-seq processes. Unlike conventional methods, EXTRAClean minimizes background noise, allowing for better representation of small RNA species. This significantly reduces unmapped reads during sequencing and increases the mapping rate of microRNAs by up to ten times.
EXTRAClean’s What is the Difference?
– Advanced Chemistry and SiC Resin Technology: The EXTRAClean series uses the same high quality SiC resin, while greatly reducing background noise due to its improved chemistry. This ensures high throughput even with samples containing particularly low amounts of RNA.
–
Capture All RNA Sizes: EXTRAClean does not discriminate based on the size of the RNA. It effectively isolates both small and long RNA fragments. This allows for a comprehensive analysis of all RNA species in liquid biopsy samples.
– Cost Effectiveness: EXTRAClean significantly reduces sequencing costs. Fewer unmapped reads and higher throughput eliminate the need for additional sequencing runs.
At NPP EXTRAClean Advantages of Using
For sensitive applications such as Next-Generation Sequencing (NGS), using EXTRAClean offers great advantages to researchers:
–
High Sensitivity:
Ensures high quality data even from small amounts of RNA samples.
–
Background
Noise
Minimization:
Clean and noise-free RNA samples provide more accurate and reliable results.
– Time and Cost Savings: Fewer sequencing runs are required, speeding up the research process and reducing costs.
Frequently Asked Questions
1. How is EXTRAClean different from its competitors?
Why should users chooseEXTRAClean? EXTRAClean offers unparalleled performance in small RNA isolation from liquid biopsy samples. By minimizing background noise, it eliminates the problems seen with other kits. This means higher throughput and lower cost.
2. What are the advantages of using EXTRAClean at NGS? Why should users chooseEXTRAClean?
EXTRAClean reduces unmapped reads in the NGS process and increases the mapping rate of microRNAs. This results in cleaner and more meaningful data, while at the same time reducing costs.
3. How is EXTRAClean different from other #Norgen #RNA kits? Has the SiCresin used been changed?
EXTRAClean uses the same high-quality SiC resin, but with improved chemistry to reduce background noise. This allows for better results, especially with samples containing low amounts of RNA.
#EXTRAClean sets a new standard in liquid biopsy and small-seq studies. For researchers looking for a cleaner, more efficient and more cost-effective solution, EXTRAClean is an indispensable option.
Letgen As Biotechnology Norgen Biotek ‘s authorized distributor in Turkey and EXTRAClean series products are available to researchers. EXTRAClean’s liquid biopsy and small RNA sequencing You can contact us to experience the superior performance it provides in its work, get detailed information about the products and start the order process.
Letgen In addition to supplying the #EXTRAClean series, we also offer technical support and consultancy services to researchers. In this way, EXTRAClean’s You can benefit from its advantages at the highest level and carry out your work more efficiently.
