While traditional epidemiological methods often focus on symptomatic individuals, asymptomatic carriers in the wider society can be overlooked. The biological traces that individuals unknowingly leave in their daily lives constitute the invisible
Wastewater offers a large, population-based, passive observation area that is beyond the reach of individual testing. Biological data collected from the entire population includes the viral load, whether symptomatic or asymptomatic. This is where the power of digital PCR technology makes a difference, as it can reliably detect even the weakest molecular signalswithin this complex matrix. In this way, it not only monitors public health, but also turns it into a source of data that can be used to generate strategic insights.
As an authorized dealer of Roche DiagnosticsLightCycler® product group, which is considered a global reference in advanced molecular technologies, Letgen Biotechnology not only offers advanced technology solutions, but also provides scientific guidance, technical consultancy and project-oriented support to researchers in the field applications of these systems. From universities to public institutions, from the private sector to environmental health, many stakeholders can find direction in accessing these advanced molecular technologies through Letgen Biotechnology.
Academic and scientific institutions that want to conduct research can request supportfrom us in the process of integrating digital PCR technology into their projects ; thus, a guiding partnership can be established in accessing and applying the technology.As Letgen Biotechnology, we are proud to be not only a product provider but also a strategic solution partner that contributes to scientific production.
As Letgen, we are able to design unique primer-probe sets and method optimization for microorganisms requested in wastewater sampling. In this way, it becomes possible to analyze the targeted pathogens with
Roche Digital LightCycler® dPCR High Sensitivity Microorganism Analysis in Wastewater with
In molecular biology, precision, reliability and versatility only make sense when they meet the right technology. At this point, Roche’s Digital LightCycler® dPCRSystem goes beyond being just a PCR platform and becomes a reference point in epidemiological surveillance.
Digital PCR is the future. And it is Roche’s engineering vision that shapes this future. Here are 6 key capabilities that differentiate this system from the competition and make it unique:
– Strong Resistance to Inhibitors: It is difficult to get reliable results in complex and inhibitor-rich samples such as wastewater. The microreaction-based system developed by Roche minimizes this obstacle and allows you to access the data directly. Molecular clarity no longer depends on sample quality.
– High Input Volume (Large Input Volume): More samples, more confidence. Thanks to the system’s high-volume analysis capacity, even low-density viral loads are not missed. This is especially critical in capturing traces from asymptomatic individuals.
– High Sensitivity (High Sensitivity): Detecting preclinical dissemination is no longer a dream. This system, which can detect even low copy numbers of nucleic acids with pinpoint accuracy, serves as a biological radar for early warning mechanisms.
– 3 Different Plate Configurations: Flexibility leads to success in the field. With three different plate options that can be optimized for different applications and sample types, the system adapts to your workflow, not your laboratory environment.
– Advanced Multiplexing with Optical Channel : The digital LightCycler® makes it possible to analyze multiple pathogens simultaneously in a single reaction. Respiratory viruses such as SARS-CoV-2, influenza A/B, RSV can all be reliably measured in the same test.
– 5X Concentrated Master Mix: This condensed formula allows you to load more samples per reaction. Result. Increased sensitivity and stronger statistical reliability.
dPCR-based wastewater surveillance is no longer just a laboratory application – it is a science-based early warning system for public health crises. It is a powerful bridge between different sectors and disciplines, putting data at the center of decision-making.
🎓 For Academic and Scientific Circles
Data sets that can analyze the burden of infection at the societal level over time and space are no longer a dream. dPCR technology enables researchers to go beyond classical modeling and conduct real-time epidemiological analyses. This means not only understanding the spread, but predicting it.
🏛️ For Public Laboratories and Health Authorities
Wastewater surveillance supported by dPCR reflects the pathogen burden in the community independently of traditional testing systems. This enables prioritized response, effective use of resources and proactive outbreak management. Gone are the days of decision-making based on intuition, not knowledge
🧬 For the Biotechnology and Environmental Health Sector
With its high sensitivity, inhibitor resistance and multiplexing capabilities, dPCR is not just an analysis method, but an R&D engine for product development, test kit design and new healthcare technologies. It also provides a strong basis for sustainability-oriented public-private collaborations.
📍 A concrete example of this multidisciplinary approach is the US Centers for Disease Control and Prevention’s (CDC) National Wastewater Surveillance System (NWSS) program. It is a global benchmark for integrating dPCR technology into public health infrastructure – and many countries are now starting to integrate similar systems into their national health policies. (See the visualization of the “dPCR-Based Wastewater Surveillance Cycle” below)
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