Increased thermal stability and hybridization specificity

Proven accurate gene identification in qPCR-dPCR assays

Ability to discriminate between alleles (SNPs) in a single pathway

Easier and more flexible designs for problematic target sequences

LNAs, referred to as ‘Locked Nucleic Acids (KNA)’ in Turkish, are useful synthetic RNA derivatives. They are also known as bridged nucleic acids (BNA). And it is often referred to as inaccessible RNA. It is bounded by a methylene bridge from 2′-oxygen and 4′-carbon atoms in the sugar-phosphate main line, and this linkage fixes the sugar ring in a 3′-endo conformation that allows the formation of hybrids complementary to DNA and RNA sequences. In this way, LNA is locked in conformation with RNA mimicry. This structure provides increased stability against enzymatic degradation. Furthermore, the structure of LNA has enhanced specificity and affinity as a component of a monomer or oligonucleotide.

LNAs are used in many real-time PCR and digital PCR applications, require no additional equipment and are compatible with all instruments. When incorporated into DNA oligonucleotides, LNA oligonucleotides offer several benefits compared to native DNA bases:

– There is less mismatch discrimination than with conventional probes.

– Incorporation into short DNA primers (< 30 nt) increases Tm by 3-8 °C for each substituted nucleotide. The increased hybridization temperature gives the sequence structural stability.

– It is widely used for specialized applications such as genotyping and transcript variant detection or differential detection of microbial species.

– The high nuclease resistance of LNAs is an important benefit for in vivo and in vitro applications. Numerous studies confirm the superior properties of LNAs as antisense agents

– There are studies with LNA in diagnostic fields.

– Immobilized LNA probes have been successfully introduced in multiplex SNP genotyping assays. This is an indication that LNAs will be much more present on the market in the future.

Biyoteknoloji ve Moleküler Çözümler

Locked Nucleic Acid (LNA)