Digital PCR (dPCR) is a technology that enables quantitative measurement of DNA or RNA samples by precisely amplifying them. dPCR is more sensitive than conventional PCR methods and is particularly effective for difficult-to-detect analyses such as rare mutations, low copy number targets and genetic variations. This technology takes place in a large number of small reaction chambers where samples are individually split so that each target molecule is analyzed independently. This results in more precise and quantitative results.
Where the Roche Digital LightCycler® differs from its competitors is its nanowell technology and large sample capacity. Nanowell plates provide smaller-volume reaction chambers during the digital PCR process, enabling more target partitioning and thus exponentially increasing sensitivity. Thanks to high sensitivity, even challenging analyses such as rare mutations or low copy number genes are completed with more reliable and reproducible results. In addition, Roche’s optimized dPCR mastermixes show superior performance even in the presence of inhibitors.
Roche’s triple nanowell plates allow each DNA or RNA molecule to be isolated and analyzed separately. These plates enable reliable analysis of genetic disorders, cancer detection, prenatal testing and rare genetic variations in clinical research. Thousands of independent reaction chambers in each plate allow a large number of targets to be processed simultaneously for faster and more efficient analysis. In addition, the small reaction volume of these plates reduces reagent usage, lowering costs and resulting in a more efficient process.
The Roche Digital LightCycler® dPCR system has become an indispensable tool for researchers and clinical laboratories by offering the most reliable solutions in sensitive genetic analysis. With its high sensitivity, inhibitor resistance and user-friendly structure, it shapes the future of dPCR technology and exhibits superior performance compared to its competitors. The advantages offered by Nanowell technology allow for faster genetic research and more precise results.
As Letgen, we enable you to achieve the most sensitive and reliable results in your projects with the Roche #Digital LightCycler® dPCR system. By using this advanced technology device in your molecular biology studies, you can increase quantification precision and data accuracy and add additional value to your projects. With our digital PCR service, we support you at all stages while optimizing your analysis processes. Contact us to use our Roche digital PCR device in your projects and discover more; let’s achieve success together!
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