{"id":3528,"date":"2025-09-03T00:01:11","date_gmt":"2025-09-02T21:01:11","guid":{"rendered":"https:\/\/letgenbio.com\/oligo-primer-resuspension-calculator\/"},"modified":"2025-10-06T18:39:59","modified_gmt":"2025-10-06T15:39:59","slug":"oligo-primer-resuspension-calculator","status":"publish","type":"page","link":"https:\/\/letgenbio.com\/en\/oligo-primer-resuspension-calculator\/","title":{"rendered":"Oligo-Primer Resuspension Calculator"},"content":{"rendered":"\t\t<div data-elementor-type=\"wp-page\" data-elementor-id=\"3528\" class=\"elementor elementor-3528 elementor-1525\">\n\t\t\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-594e9fa ts-col-stretched-none ts-bg-color-over-image elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"594e9fa\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-top-column elementor-element elementor-element-14f7d9d ts-bg-color-over-image\" data-id=\"14f7d9d\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-f891f98 elementor-widget elementor-widget-html\" data-id=\"f891f98\" data-element_type=\"widget\" data-widget_type=\"html.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<!-- LETGENBIO OLIGO RESUSPENSION CALCULATOR START --><div id=\"letgenbio-oligo-calculator\"><style>#letgenbio-oligo-calculator {\n            background-color: #f4f9f4;\n            border-radius: 12px;\n            padding: 25px;\n            box-shadow: 0 4px 12px rgba(0, 0, 0, 0.08);\n            font-family: -apple-system, BlinkMacSystemFont, \"Segoe UI\", Roboto, Helvetica, Arial, sans-serif;\n            color: #333;\n            max-width: 500px;\n            margin: 20px auto;\n            border: 1px solid #dce9e3;\n        }\n        #letgenbio-oligo-calculator h3 {\n            margin: 0 0 20px 0;\n            font-size: 22px;\n            color: #04724d;\n            text-align: center;\n            display: flex;\n            align-items: center;\n            justify-content: center;\n        }\n        #letgenbio-oligo-calculator h3 svg {\n            margin-right: 10px;\n        }\n        #letgenbio-oligo-calculator .calc-section {\n            margin-bottom: 20px;\n        }\n        #letgenbio-oligo-calculator .calc-section-title {\n            font-weight: 600;\n            margin-bottom: 10px;\n            font-size: 16px;\n            color: #04724d;\n        }\n        #letgenbio-oligo-calculator .input-group {\n            display: flex;\n            align-items: center;\n            margin-bottom: 15px;\n        }\n        #letgenbio-oligo-calculator .input-group label {\n            flex: 0 0 140px;\n            font-weight: 500;\n            font-size: 15px;\n            padding-right: 10px;\n        }\n        #letgenbio-oligo-calculator .input-field {\n            flex: 1 1 auto;\n            display: flex;\n        }\n        #letgenbio-oligo-calculator .input-field input[type=\"number\"],\n        #letgenbio-oligo-calculator .input-field select {\n            border: 1px solid #ced4da;\n            padding: 10px;\n            font-size: 16px;\n            width: 100%;\n            transition: border-color 0.2s, box-shadow 0.2s;\n            background-color: #fff;\n        }\n        #letgenbio-oligo-calculator .input-field input[type=\"number\"] {\n            border-radius: 8px 0 0 8px; border-right: none; -moz-appearance: textfield;\n        }\n        #letgenbio-oligo-calculator .input-field input[type=\"number\"]::-webkit-outer-spin-button,\n        #letgenbio-oligo-calculator .input-field input[type=\"number\"]::-webkit-inner-spin-button {\n            -webkit-appearance: none; margin: 0;\n        }\n        #letgenbio-oligo-calculator .input-field select {\n            border-radius: 0 8px 8px 0; cursor: pointer; background-color: #e9ecef;\n        }\n        #letgenbio-oligo-calculator .input-field input[type=\"number\"]:focus {\n            outline: none; border-color: #04724d; box-shadow: 0 0 0 2px rgba(4, 114, 77, 0.2); position: relative; z-index: 1;\n        }\n        #letgenbio-oligo-calculator .mass-options {\n            background-color: #fff; border: 1px solid #dce9e3; border-radius: 8px; padding: 15px; margin-top: -5px; margin-bottom: 15px;\n            max-height: 0; overflow: hidden; transition: max-height 0.5s ease-out, padding 0.5s ease-out, opacity 0.5s ease-out;\n            opacity: 0; padding-top: 0; padding-bottom: 0;\n        }\n        #letgenbio-oligo-calculator .mass-options.visible {\n            max-height: 300px; opacity: 1; padding: 15px;\n        }\n        #letgenbio-oligo-calculator .radio-group-mw { display: flex; gap: 20px; margin-bottom: 15px; }\n        #letgenbio-oligo-calculator .radio-group-mw label { display: flex; align-items: center; gap: 5px; cursor: pointer; }\n        #letgenbio-oligo-calculator .mw-input-area textarea {\n            width: 100%; padding: 10px; border-radius: 8px; border: 1px solid #ced4da; font-family: 'Courier New', Courier, monospace;\n            font-size: 14px; resize: vertical; min-height: 80px;\n        }\n        #letgenbio-oligo-calculator .mw-input-area .mw-display { font-size: 14px; color: #555; margin-top: 5px; text-align: right; }\n        #letgenbio-oligo-calculator .mw-input-area .mw-display span { font-weight: bold; color: #000; }\n        #letgenbio-oligo-calculator .result-section {\n            background-color: #dce9e3; border: 1px solid #99c1b9; border-radius: 8px; padding: 20px; text-align: center; margin-top: 20px;\n        }\n        #letgenbio-oligo-calculator .result-title {\n            font-size: 16px; color: #04724d; margin: 0 0 10px 0; font-weight: 600;\n        }\n        #letgenbio-oligo-calculator .result-value {\n            font-size: 28px; font-weight: 700; color: #023c2a; word-wrap: break-word;\n        }\n        #letgenbio-oligo-calculator .error-message {\n            color: #ae2012; font-size: 14px; text-align: center; min-height: 20px; margin-top: 15px;\n        }\n        #letgenbio-oligo-calculator .hidden { display: none; }\n    <\/style><h3><svg xmlns=\"http:\/\/www.w3.org\/2000\/svg\" width=\"24\" height=\"24\" viewBox=\"0 0 24 24\" fill=\"none\" stroke=\"currentColor\" stroke-width=\"2\" stroke-linecap=\"round\" stroke-linejoin=\"round\"><path d=\"M21 15c0-4.4-3.6-8-8-8s-8 3.6-8 8\"><\/path><path d=\"M10 10c0-3.3 1.8-6 4-6\"><\/path><path d=\"M12 12c0-2.8.9-5 2.5-5\"><\/path><path d=\"M14 14c0-1.7.4-3 1-3\"><\/path><path d=\"M3 21h18\"><\/path><path d=\"M5 21V10.5\"><\/path><path d=\"M19 21V10.5\"><\/path><\/svg>Oligo\/Primer Resuspension\n    <\/h3><div class=\"calc-section\"><div class=\"input-group\"><label for=\"oligo-amount\">Oligo Amount<\/label><div class=\"input-field\"><input type=\"number\" id=\"oligo-amount\" placeholder=\"e.g., 25\"><select id=\"oligo-unit\"><option value=\"nmol\" selected>nmol<\/option><option value=\"pmol\">pmol<\/option><option value=\"ug\">\u00b5g<\/option><\/select><\/div><\/div><div class=\"mass-options\" id=\"mass-options\"><div class=\"radio-group-mw\"><label><input type=\"radio\" name=\"mw-method\" value=\"sequence\" checked> Calculate from Sequence<\/label><label><input type=\"radio\" name=\"mw-method\" value=\"manual\"> Enter MW Manually<\/label><\/div><div class=\"mw-input-area\"><div id=\"sequence-input-area\"><textarea id=\"oligo-sequence\" placeholder=\"Paste DNA sequence here (e.g., ATGC...)\"><\/textarea><div class=\"mw-display\">Calculated MW: <span id=\"calculated-mw\">-<\/span> g\/mol<\/div><\/div><div id=\"manual-mw-input-area\" class=\"hidden\"><div class=\"input-field\"><input type=\"number\" id=\"manual-mw-value\" placeholder=\"Molecular Weight (g\/mol)\"><\/div><\/div><\/div><\/div><div class=\"input-group\"><label for=\"final-conc\">Final Concentration<\/label><div class=\"input-field\"><input type=\"number\" id=\"final-conc\" value=\"100\" placeholder=\"e.g., 100\"><select id=\"final-conc-unit\"><option value=\"uM\" selected>\u00b5M<\/option><option value=\"mM\">mM<\/option><option value=\"nM\">nM<\/option><\/select><\/div><\/div><\/div><div class=\"error-message\" id=\"error-message\"><\/div><div class=\"result-section\"><div class=\"result-title\">Required Diluent Volume<\/div><div class=\"result-value\" id=\"result-value\">-<\/div><\/div><script>(function() {\n            const calculator = document.getElementById('letgenbio-oligo-calculator');\n            if (!calculator) return;\n\n            const oligoAmount = calculator.querySelector('#oligo-amount');\n            const oligoUnit = calculator.querySelector('#oligo-unit');\n            const finalConc = calculator.querySelector('#final-conc');\n            const finalConcUnit = calculator.querySelector('#final-conc-unit');\n            const resultValue = calculator.querySelector('#result-value');\n            const errorMessage = calculator.querySelector('#error-message');\n            \n            \/\/ Extra fields for \u00b5g\n            const massOptions = calculator.querySelector('#mass-options');\n            const mwMethodRadios = calculator.querySelectorAll('input[name=\"mw-method\"]');\n            const sequenceInputArea = calculator.querySelector('#sequence-input-area');\n            const manualMwInputArea = calculator.querySelector('#manual-mw-input-area');\n            const oligoSequence = calculator.querySelector('#oligo-sequence');\n            const calculatedMwDisplay = calculator.querySelector('#calculated-mw');\n            const manualMwValue = calculator.querySelector('#manual-mw-value');\n\n            \/\/ Average nucleotide weights for ssDNA\n            const mwBases = { 'A': 313.2, 'T': 304.2, 'C': 289.2, 'G': 329.2 };\n            const mwPhosphodiesterAdjustment = 61.96;\n\n            const unitFactors = {\n                amount: { nmol: 1, pmol: 1e-3, ug: null }, \/\/ \u00b5g needs special handling\n                concentration: { uM: 1, mM: 1e3, nM: 1e-3 }\n            };\n\n            function calculateMW(sequence) {\n                sequence = sequence.toUpperCase().replace(\/[^ATCG]\/g, '');\n                if (sequence.length === 0) return 0;\n                let totalMW = 0;\n                for (const base of sequence) {\n                    if (mwBases[base]) totalMW += mwBases[base];\n                }\n                totalMW -= (sequence.length - 1) * mwPhosphodiesterAdjustment;\n                return totalMW > 0 ? totalMW : 0;\n            }\n\n            function calculate() {\n                try {\n                    errorMessage.textContent = '';\n                    const amount = parseFloat(oligoAmount.value);\n                    const concentration = parseFloat(finalConc.value);\n\n                    if (isNaN(amount) || isNaN(concentration)) {\n                        resultValue.textContent = '-';\n                        return;\n                    }\n                    if (amount < 0 || concentration <= 0) {\n                       throw new Error(\"Values must be positive (concentration > 0).\");\n                    }\n\n                    let amountInNmol;\n                    const selectedAmountUnit = oligoUnit.value;\n                    \n                    if (selectedAmountUnit === 'ug') {\n                        const mwMethod = calculator.querySelector('input[name=\\\"mw-method\\\"]:checked').value;\n                        let mw = 0;\n                        if(mwMethod === 'sequence') {\n                           mw = calculateMW(oligoSequence.value);\n                           calculatedMwDisplay.textContent = mw > 0 ? formatNumber(mw) : '-';\n                        } else {\n                           mw = parseFloat(manualMwValue.value);\n                           calculatedMwDisplay.textContent = '-';\n                        }\n\n                        if (isNaN(mw) || mw <= 0) {\n                            throw new Error(\"For \u00b5g unit, enter a valid sequence or a positive molecular weight.\");\n                        }\n                        \/\/ \u00b5g -> g -> mol -> nmol\n                        amountInNmol = (amount * 1e-6 \/ mw) * 1e9;\n                    } else {\n                        amountInNmol = amount * (unitFactors.amount[selectedAmountUnit] || 1);\n                    }\n                    \n                    const concentrationInUm = concentration * (unitFactors.concentration[finalConcUnit.value] || 1);\n\n                    \/\/ V (\u00b5L) = (nmol * 1000) \/ \u00b5M\n                    const volumeInUl = (amountInNmol * 1000) \/ concentrationInUm;\n                    \n                    if (isNaN(volumeInUl) || !isFinite(volumeInUl)) {\n                        resultValue.textContent = '-';\n                    } else {\n                        resultValue.textContent = `${formatNumber(volumeInUl)} \u00b5L`;\n                    }\n\n                } catch (error) {\n                    errorMessage.textContent = error.message;\n                    resultValue.textContent = '-';\n                }\n            }\n            \n            function updateUI() {\n                if (oligoUnit.value === 'ug') {\n                    massOptions.classList.add('visible');\n                } else {\n                    massOptions.classList.remove('visible');\n                }\n                const mwMethod = calculator.querySelector('input[name=\\\"mw-method\\\"]:checked').value;\n                if(mwMethod === 'sequence') {\n                    sequenceInputArea.classList.remove('hidden');\n                    manualMwInputArea.classList.add('hidden');\n                } else {\n                    sequenceInputArea.classList.add('hidden');\n                    manualMwInputArea.classList.remove('hidden');\n                }\n                calculate();\n            }\n\n            function formatNumber(num) {\n                if (num === 0) return 0;\n                if (Math.abs(num) < 1e-3) return num.toExponential(3);\n                if (Math.abs(num) >= 1e6) return num.toExponential(3);\n                return parseFloat(num.toPrecision(5));\n            }\n            \n            [oligoAmount, finalConc, oligoSequence, manualMwValue].forEach(el => el.addEventListener('input', calculate));\n            [oligoUnit, finalConcUnit].forEach(el => el.addEventListener('change', updateUI));\n            mwMethodRadios.forEach(radio => radio.addEventListener('change', updateUI));\n            \n            updateUI();\n        })();\n    <\/script><\/div><!-- LETGENBIO OLIGO RESUSPENSION CALCULATOR END -->\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<\/div>\n\t\t","protected":false},"excerpt":{"rendered":"<p>Oligo\/Primer Resuspension Oligo Amountnmolpmol\u00b5g Calculate from Sequence Enter MW ManuallyCalculated MW: &#8211; g\/molFinal Concentration\u00b5MmMnMRequired Diluent Volume-<\/p>\n","protected":false},"author":1,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"elementor_header_footer","meta":{"inline_featured_image":false,"footnotes":""},"class_list":["post-3528","page","type-page","status-publish","hentry"],"_links":{"self":[{"href":"https:\/\/letgenbio.com\/en\/wp-json\/wp\/v2\/pages\/3528","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/letgenbio.com\/en\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/letgenbio.com\/en\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/letgenbio.com\/en\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/letgenbio.com\/en\/wp-json\/wp\/v2\/comments?post=3528"}],"version-history":[{"count":0,"href":"https:\/\/letgenbio.com\/en\/wp-json\/wp\/v2\/pages\/3528\/revisions"}],"wp:attachment":[{"href":"https:\/\/letgenbio.com\/en\/wp-json\/wp\/v2\/media?parent=3528"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}